background:  stresses such as heat shock, starvation, or osmotic is essential to lead isolated microspores towards embryogenesis. despite the effectiveness of stresses in embryogenesis, they exert adverse effects on metabolism and growth of the regenerated plants.objectives: the effects of heat shock and 2,4-d treatment on total protein content of treated microspores, morphological and physiolo...

In maize, doubled haploid (DH) technology is used in breeding programs to obtain lines, the primary input for developing new cultivars. This review aims present main points related using this steps its application, and advantages implications of DH lines maize programs.

Doubled haploids (DH) have become a powerful tool to assist in different basic research studies, and also applied research. The principal (but not the only) routine use of DH by breeding companies is produce pure lines for hybrid seed production crop species. Several decades after discovery haploid inducer maize anther culture as method plants from pollen precursors, biotechnological revolution...

The needs of a growing human population require rapid and efficient development of improved cultivars by plant breeders. The doubled haploid (DH) technology enables generating completely homozygous lines in a single step and, thus, is central to modern genetics and breeding approaches. Rapid and reliable identification of seeds with a haploid embryo after in vivo haploid induction is elementary...

The production of maize doubled haploid (DH) lines is a technique commonly used by private companies, but not by Brazilian public institutions. Research on this technique is essential to develop and improve the production of DH lines grown under tropical conditions. We assessed the ability of a gynogenetic haploid inducer system to induce haploids in a tropical environment, assessed the inducti...

with increasing the world population, and needing to feed it, the yield enhancement of cereals crops is of the most important approaches meet this target, which is achieved via identifying the effective factors underlay yield of cereals like wheat. this experiment was conducted to evaluate the grain yield and its association with some contributed traits in a doubled-haploid (dh) wheat populatio...

The importance of haploid and doubled haploid (DH) techniques for basic and applied research, as well as to improve the speed of genetic gain when applied in breeding programs, cannot be overstated. They have become routine tools in several major crop species, such as maize (Zea mays L.), wheat (Triticum aestivum L.), and barley (Hordeum vulgare L.). DH techniques in perennial ryegrass (Lolium ...

Background:  Stresses such as heat shock, starvation, or osmotic is essential to lead isolated microspores towards embryogenesis. Despite the effectiveness of stresses in embryogenesis, they exert adverse effects on metabolism and growth of the regenerated plants. Objectives: The effects of heat shock and 2,4-D treatment on total protein content of treated microspores, morphological and physiol...

Abstract Diverse processes leading to doubled haploid (DH) plant production, such as microspore embryogenesis, gynogenesis, and distant hybridization followed by genome elimination, are based on the unique ability of cells form embryos without fertilization. All these possible because various in vitro culture systems that enable growth development tissues or single outside parental organism. Th...